Ogni antibiotico è efficace in relazione a un determinato gruppo di microrganismi comprare keflex senza ricettain caso di infezioni oculari vengono scelte gocce ed unguenti.

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Exertional myopathy post fox attack in an agile wallaby (Macropus agilis).
1.1 Introduction
Exertional myopathy or ‘capture myopathy' refers to a non-infectious, metabolic syndrome characterized by damage to skeletal or cardiac muscle following intense physical activity. Skeletal muscle necrosis and acute renal failure are common sequelae (Hartup et al. 1999). In mammals, the disease has been primarily documented in cervids, (Wallace et al. 1987) macropods, cetaceans, crocodilians, pinnipeds, rodents and primate species (Hartup et al. 1999). Elevated environmental temperature may increase the likelihood of the disease and in macropods the disease is most commonly associated with capture or restraint procedures (Chalmers & Barrett 1982, Spraker 1993, Williams & Thorne 1996), repeated handling, close confinement or exposure to predators (Vogelnest & Portas 2008). In severe cases, death may occur, acutely within hours, or more commonly a sub-acute syndrome of mortality between two and four days after the onset of clinical signs is reported (Carpenter 1993). Chronically affected animals may experience a delayed-peracute syndrome causing death weeks later, and individuals if only mildly affected then exposed to repeated stress may relapse and develop the acute form of disease (Hartup et al. 1999). Clinical history
A five year old, female, Agile wallaby (Macropus agilis) A00035 in a zoological collection presented with wet forearms from overgrooming, restlessness, frequent head movements, a stumbling gait, and appeared anxious and agitated in demeanor. A carcass of a bridle nail tailed wallaby (Onychogalea fraenata) was found in close proximity which appeared to have been predated upon by a fox owing to the primary wound and lack of musculature surrounding the jugular vein and lateral neck (Figure 1). A fox had also been sighted exiting the zoo grounds the night prior. This wallaby was housed in an open bushwalk enclosure, approximately 200m by 200m in diameter. Five agile wallabies, and ten red (Macropus rufus) and Western grey (Macropus fulginosus) kangaroos also resided in this non-fenced area. Two other male agile wallabies, appeared skittish, but did not appear as agitated as A00035.


Figure 1. Typical lesions of predation by the introduced European red fox
(Vulpes vulpes
). The site of attack is the lateral neck surrounding the jugular,
and bite wounds and tearing through the muscular regions of the neck is noted
with a 22 mm inter-canine distance.

Clinical examination
The gait and demeanor of this wallaby were observed from behind a tree with binoculars as to not initiate a further flight response. Increased activity with associated stumbling and unsteadiness was seen. Wide dilated pupils with ears pointed in a backward direction were evident and respiratory rate was increased (40rpm). Given the keepers had noticed this behaviour over the past hour prior to calling for veterinary attention a decision was made to anaethetise the wallaby immediately for diagnosis, treatment and cooling. Supportive care
The sprinklers were immediately turned on to cool the wallaby, and the bushwalk area closed to the public to minimize any further excitation. The two other affected agile wallabies of an estimated 11.5kg were darted with the neuroleptic azaperone (Stresnil® Boehringer Ingelheim Pty. Ltd, NSW Australia) 2mg/kg IM and fluphenazine (Modecate® Bristol-Myers Squibb, NSW Australia) 2.5mg/kg IM. (Appendix 2). This was to ensure rapid and sustained tranquilisation. Keepers conducted hourly checks of all the macropods in the bushwalk. Diagnostic procedures
The wallaby was darted with a CO2 powered pistol (Tel-Inject Australasia®, Victoria Australia) from an approximate distance of 8 meters with 7mg/kg of tiletamine/zolazepam (Zoletil® Virbac, Australia) (Appendix 1). Eight minutes post darting this wallaby was in lateral recumbency and a hand towel was placed over the eyes to minimize further excitation. At 10 minutes post darting the wallaby was judged to be adequately sedated for transport, there was no corneal reflex or spontaneous movement elicited on palpation with a broom handle. A stretcher was slid beneath the wallaby's body for transport to the veterinary hospital.


Once at the veterinary department the wallaby was placed on a face mask delivering 5% isoflurane and 2L/minute oxygen and her temperature taken (38'C) then intubated as soon as jaw tone had decreased. (Figure 2). Figure 2.
Intubation of an agile wallaby with the aid of a laryngoscope and stylet
A 22G catheter was inserted into the lateral caudal vein and 5mL of blood drawn. Packed cell volume (PCV) and total protein (TP) was measured on a refractometer. The remaining blood was submitted for a complete blood count (CBC), biochemistry, and serum storage. Intravenous fluids (0.9% NaCl solutiom, Baxter healthcare, NSW Australia) were commenced at 10ml/kg/hr. An elevated PCV and TP indicated likely haemoconcentration from Despite being anaethetised spontaneous muscle activity and fasciculations were noted in the hindlimbs. However jaw tone and cloacal tone was absent indicative of deep sedation, the muscle fasciculation's were more likely related to the myonecrotic state. There was no evidence of trauma on clinical exam. Temperature was beginning to decrease (37'C) and the respiratory rate and heart rate had stabilized at 20rpm and120bpm respectively. Mucous membranes were tacky. The bladder was palpated and a patch of hair shaved directly above the bladder. This site was aseptically prepared and cystocentesis was conducted. The urine obtained was pink in colouration with +++ blood found on urine dipstix (Figure 3).


Figure 3.
Blood was evident on cystocentesis.
Radiographs were taken of the pelvis and hindlimbs for the evidence of fractures or bony pathology, no abnormalities were detected. Differential diagnoses included; nutritional, toxic, infectious and parasitic myopathies however given the history the most likely diagnoses was that of an exertional myopathy occurring in response to a fox sighting. Treatment
Once anaethetised the isoflurane was dropped to 1.5%, however the oxygen remained at 2L/min to promote oxygenation of muscle and enhance tissue perfusion. Diuresis and cooling was undertaken through IV fluid therapy. Body temperature was closely monitored and controlled with cool packs, fans, mist and IV fluids. Muscle fasciculation's and myonecrosis was reduced with the skeletal muscle relaxant diazepam (Pamlin®, Parnell Laboratories, NSW Australia) administered IV 0.5-1 mg/kg (Rose 1999). Dantrolene (Dantrium powder for injection®, Pfizer, NSW, Australia) a skeletal muscle relaxant used in humans for treatment of malignant hyperthermia was administered slowly IV at 1mg/kg then 1mg/kg PO SID 3-5 days. Prednisolone sodium succinate (Solu-Delta Cortef powder®, Pharmacia & Upjohn Company, Michigan USA) (5-10mg/kg IV) was administered followed by Vitamin E/Selenium (Selevite®, Troy Laboratories Pty Ltd, NSW Australia) 1ml/50kg body weight. The isoflurane saturation was slowly reduced and the endo tracheal tube removed once swallowing evident. Azaperone was administered 0.5mg/kg IM to provide short term tranquilisation and sedation. The wallaby was placed in a hessian sack in lateral recumbency in a large dog pet pack with a maintenance dose of IV fluids being delivered through the catheterized lateral tail vein. The wallaby was then moved to a quiet, dark, recovery room within the veterinary hospital for observation. The wallaby was rolled every two hours to prevent respiratory deficits and pressure necrosis. Recovery was prolonged and there was little attempt to stand until six hours post-anaesthetic. Blood results revealed massively


elevated muscle enzymes, and evidence of severe renal compromise (CK > 20 000 U/L, significantly elevated ALP, AST, urea and creatinine). The wallaby was severely depressed, had minimal response to stimuli, had developed a fever (38°C) and gurgly respiration possibly indicative of pulmonary oedema and was given a poor prognosis. 1.3 Outcome
A decision was made to euthanse this animal on medical and welfare grounds post consultation with curatorial staff. Given the wallaby was already deeply sedated 5mL of IV pentobarbitone (Lethabarb euthanasia injection®, Virbac Australia Pty Ltd, NSW Australia) was delivered through the lateral caudal vein and death shortly followed. A post mortem conducted revealed a paleness of the skeletal muscle especially of the femoral and gluteal muscles, and muscular haemorrhage (Figure 5). On histopathology haemorrhage and oedema throughout the interstitium of the hindlimb adductor muscles and multifocal myofibre degeneration was reported. Renal changes consisted of hydropic change, and necrosis of renal tubule epithelial cells, with pulmonary congestion and oedema (S.Wong, personal communication, 2009). No infectious causes were found on culture of routine tissues and histopathology. The body was disposed of in the quarantine bins then incinenerated to prevent the potential for residue and secondary poisonings. Figure 5.
Typical lesions of an exertional myopathy were evident including
paleness of the femoral muscles and muscular haemorrhage.
Discussion
Capture myopathy occurs as a result of prolonged sympathetic nervous system stimulation causing ischaemia as a result of reduced tissue perfusion, lactic acidosis and muscular adenosisne triphosphate reserve depletion. Which in turn may lead to cardiovascular and circulatory collapse, muscular compartment syndrome and acute renal failure subsequent to ischaemia and myoglobinuric nephrosis (Vogelnest & Portas 2008). In this case the most likely stimulator was the sighting of a predator which led to the above cascade of events and eventual demise. The blood in the urine most likely occurred due to myoglobinuric nephrosis. Whereby the severe muscle breakdown lead to a circulating myoglobinemia. Myoglobin is toxic to the proximal tubules and loop of Henle (Rose 2005) and this combined with the associated circulatory collapse contributes to the acute tubular necrosis seen in this condition. Fluid therapy with 0.9% NaCl is a mainstay of treatment for many reasons, to improve perfusion to the kidney, dilute the damage that myoglobin causes to the kidney, dilute the lactic acid in the blood stream, and so improve heart function, expand the blood volume and so address the mechanisms of shock, and reverse the hyperthermia. Steroids were administered as they may contribute to stabilising cell membranes to prevent ongoing or irreversible cell degeneration and Vitamin E and Selenium were administered as it has been proven in some species to reduce the risk of subsequent myopathy (Williams & Thorne 1996). The biochemical changes that occurred are related to metabolic acidosis and include elevation in muscle enzymes (CK, AST & LDH). The CK level increases rapidly in response to cardiac or skeletal muscle damage, yet owing to the enzyme's short half life will only remain highly elevated if ongoing muscle damage is occurring (Fowler 1993). AST has a longer half life than CK. As such we conducted serial monitoring of AST & CK to provide insight into the duration and degree of muscle damage which was sub-acute and had most likely had begun the night before presentation in response to the fox sighting. The prevention of capture myopathy is far more effective than the cure, and prophylactic measures should be considered every time a susceptible species is handled or anaesthetised. In this case, the stimulus was difficult to prevent, although fencing has subsequently been improved by adding further height and digging the wire three feet beneath the ground to prevent tunneling of predators. When anaethetising a susceptible species, noise and movement should be kept to a minimum during induction and elective procedures should be conducted in the cool of the day (early morning) and appropriate restraint for the size of the animal should be considered. In the event of a stressful situation, it may be useful to administer neuroleptic drugs prophylactically. Conclusion
This agile wallaby presented with clinical signs of overgrooming, agitated behaviour and a stumbling gait. CBC and biochemistry indicated haemoconcentration, severe myonecrosis and azotaemia and cystocentesis found blood in the urine, all consistent with a diagnosis of an exertional myopathy when combined with the history of a fox attack and sighting the night previously. Treatment was attempted to reverse concurrent shock and hyperthermia, reverse metabolic acidosis, and stabilise cellular membranes. However owing to the severity of the myopathy, on repeated blood sampling and advancing clinical deterioration a poor prognosis for recovery was given and the wallaby euthansed. Lesions consistent with acute tubular nephrosis, and skeletal muscle necrosis were evident histologically. Fencing upgrades to minimize further predation and the provision of neuroleptics to other exposed macropods have prevented further deaths. References
Chalmers GA, & Barrett MW, Capture myopathy In: Noninfectious diseases of Wildlife. GL. Hoff & JW. Davis (eds.). Iowa State University Press, Ames, Iowa, Pp. 84–94. Fowler MF (1993) Zoo and wild animal medicine, current therapy 3, W.B Saunders. Pennsylvania USA. Hartup BK, Koillas GV, Jacobsen MC, Valentine BA, & Kimber KK. Exertional myopathy in
translocated river otters from New York. Journal of Wildlife Diseases. 1999; 35(3): 542–547
Rose K. (2005) Common disease of Urban Wildlife. Wildlife in Australia: Healthcare & Management, General diseases – myopathy & trauma. Australian Registry of Wildlife Health Field Manual. Pp. 1-11. Spraker TR. Stress and capture myopathy in artiodactylids. In: Zoo and wild animal medicine, current therapy 3, ME. Fowler (ed.). W. B. Saunders Company, Philadelphia, Pennsylvania. Pp. 481–488. Vogelnest L, & Portas T (2008) Macropods. In: Medicine of Australian Mammals. L. Vogelnest, R. Woods (eds). CSIRO Publishing, Sydney, Australia.
Wallace RS, Bush M, & Montali RJ Deaths from exertional myopathy at the National
Zoological Park from 1975 to 1985. Journal of Wildlife Diseases. 1987; 23(3): 454-462
Williams ES, & Thorne ET (1996) Exertional myopathy. In: Non-infectious diseases of
wildlife.
2nd Ed. A. Fairbrother, LN. Locke, GL Hoff (eds). Iowa State Press, Ames, Iowa.
Pp. 181 - 193.
Appendix 1:
Agile Wallaby A00035
EUA, suspected exertional Procedure
Veterinary:
Drug Conc
Total dose
Total dart
volume ml
Zolazepam (Zoletil) 1mg/mL Drug Conc
Total dose
volume ml
Drug Conc
Total dose
Route Notes
volume ml
Adrenaline 1:1,000 0.447 0.745 IV/SC Diazepam 0.5-1mg/kg CV/Resp:
Integument:
Urogenital:
cystocentesis express Blood tests:
ZP1, serum banking, toxoplasmosis Rectal swab:


Appendix 2: Neuroleptic drug combinations
Generic Name
Duration
(Stresnil® Boehringher Haloperidol B.P. (Serenace® Searl) Duration up to 4 weeks Duration 3-4 weeks From: Vogelnest, L. (1998) Chemical Restraint of Native Fauna Wildlife Proceedings 1998. pp.150-187

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LABORATOIRE DE BIOLOGIE MÉDICALE BIOMEDYS 509 avenue du 8 Mai 1945 69300 CALUIRE ET CUIRE Le MANUEL DE PRELEVEMENT regroupe les indications, consignes et informations nécessaires à la prise en charge des patients lors de l'acte de prélèvement. Il est destiné aux préleveurs du laboratoire, aux préleveurs externes et aux laboratoires transmetteurs.